Establishment of SYBR Green I Real-Time PCR for Detection of <i>Streptococcus agalactia</i>e in Aquaculture Waters

<div>Specificity analysis of the real-time PCR using SYBR Green I. Curve 1: total DNA genome isolated from <i>S. agalactiae</i> DNA. Curves 2–5: total DNA genome isolated from <i>Aeromonas veronii, Staphylococcus epidermidis, Aeromonas hydrophila</i>, and <i>Edwardsiella tarda,</i> respectively. Curve 6: negative control without template. The primer <i>cfb</i>-qF/R was used for the specificity analysis.</div>

Journal of Applied Ichthyology

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Figure 3: Establishment of SYBR Green I Real-Time PCR for Detection of <i>Streptococcus agalactia</i>e in Aquaculture Waters 

Journal of Applied Ichthyology

Establishment of SYBR Green I Real-Time PCR for Detection of Streptococcus agalactiae in Aquaculture Waters

Figure 3