Establishment of SYBR Green I Real-Time PCR for Detection of Streptococcus agalactiae in Aquaculture Waters

Figure 4

Sensitivity analysis of the real-time PCR using SYBR Green I. Amplification curve of real-time PCR for detection of tenfold serial dilutions of recombinant plasmid pMD18-T-cfb after 40 cycles. Curves 1–6: 1.57 × 10⁷, 1.57 × 10⁶, 1.57 × 10⁵, 1.57 × 10⁴, 1.57 × 10³, and 1.57 × 10² copies/μL pMD18-T-cfb, respectively. Curve 7: 1.57 × 10¹ copies/μL pMD18-T-cfb. Curve 8: negative control without template. The primer cfb-qF/R was used for the specificity analysis.