Establishment of SYBR Green I Real-Time PCR for Detection of Streptococcus agalactiae in Aquaculture Waters

Figure 5

Determination of the gene cfb copy number of S. agalactiae in aquaculture waters. (a) Amplification curve of Liuzhou water sample using real-time PCR method. Liuzhou water sample was concentrated by 3000 folds. (b) Amplification curve of Shatang water sample using real-time PCR method. Shatang water sample was concentrated 2000 folds. The red baseline and the gray baseline are negative controls. The concentrations of DNA were 81.176 ng/μl and 75.487 ng/μl used for Liuzhou and Shatang, respectively. The primer cfb-qF/R was used for the specificity analysis. All experiments were repeated three times.

(a)

(b)