Establishment of SYBR Green I Real-Time PCR for Detection of Streptococcus agalactiae in Aquaculture Waters

Table 2

Repeatability and stability test of real-time PCR assay.

Template concentration of standard (copies/μL)	Intra-group repetition		Inter-group repetition
	Mean ± SD^a	CV (%)^b	Mean ± SD^a	CV (%)^b
1.57  10^6	16.96 ± 0.05	0.30	16.81 ± 0.12	0.69
1.57  10^5	21.76 ± 0.12	0.55	21.24 ± 0.37	1.75
1.57  10^4	24.41 ± 0.24	0.99	24.31 ± 0.22	0.90

^aCalculation of copy number was based on average Ct values. ^bCV is the coefficient of variation, which is a normalized measure of the dispersion of probability distribution. It is defined as the ratio of standard deviation to average, that is, CV = (standard deviation/average MEAN) × 100%.