Molecular Characterization, Phylogeny, and Expression Profiles of SoxE Subfamily in Scophthalmus maximus

Table 2

Primers for qRT-PCR.

Gene name	Primer sequence (5′-3′)	Tm (°C)	Amplicon length (bp)
β-actin	F: TGA​ACC​CCA​AAG​CCA​ACA​GG	60	107
	R: AGA​GGC​ATA​CAG​GGA​CAG​CAC
Sox8a	F: CCC​GCC​ACA​GAC​CTC​AGA​TA	60	147
	R: AAG​ACG​ACG​AGG​AGG​AGG​TG
Sox8b	F: CGC​AGC​TTC​TTT​CTC​CAG​CA	60	129
	R: TAG​GGC​CGT​CTG​GAT​GAG​TG
Sox9a	F: CTG​CCG​TTT​TCT​GTC​CAC​TTC	60	204
	R: TCT​TGT​TCC​TCC​GTC​ATC​TTC
Sox9b	F: CCG​GGA​GAA​CAC​TCA​GGT​CA	60	87
	R: CAG​GTC​AGC​TTT​GCT​GGA​GG
Sox10a	F: ACA​CAG​AGC​AGG​CAT​TCA​TAC​TTG​G	60	87
	R: GAA​TCC​GTG​AGT​TCC​CGA​GTT​TCC
Sox10b	F: ATC​AGA​GAA​GCG​GTC​AGT​CAG	60	288
	R: GTC​CTT​CTT​GTG​CTG​TTT​CCT