Effects of Varying Dietary Protein Levels on Growth Performance, Survival, Body Composition, Haemato-Biochemical Profile, and Metabolic Responses of Hypselobarbus jerdoni (Day, 1870) Juveniles

Table 1

Formulation and proximate composition of the experimental diets (g/kg, dry matter basis).

Composition ingredients (g/kg)	Treatments^a
	TCP20	TCP25	TCP30	TCP35	TCP40	TCP45
GNOC^b	205	205	205	205	205	205
Soybean meal	205	205	205	205	205	205
Casein	10	56	102	148	194.8	242
Gelatin	2.5	14	25.5	37	48.7	60.5
Starch	360	320	280	240	200	160
Dextrin	129.4	111.9	94.4	76.9	58.4	39.4
Fish oil	30	30	30	30	30	30
Veg oil	17.5	17.5	17.5	17.5	17.5	17.5
Vit. min. mix^c	15	15	15	15	15	15
CMC^d	20	20	20	20	20	20
BHT^e	0.2	0.2	0.2	0.2	0.2	0.2
Betaine	5	5	5	5	5	5
Stay-C^f	0.3	0.3	0.3	0.3	0.3	0.3
Vitamin E	0.1	0.1	0.1	0.1	0.1	0.1
Total	1000	1000	1000	1000	1000	1000
Proximate composition (g/kg; on dry matter basis; mean of triplicates)
Moisture	97.33	95.16	92.07	94.00	91.10	99.16
Crude protein	200.53	251.53	301.43	350.17	401.77	451.90
Ether extract	59.42	58.47	58.53	60.40	59.77	61.27
Total ash	43.10	40.90	40.17	45.27	45.20	48.60
Crude fibre	21.47	21.67	21.80	22.50	21.67	21.00
Nitrogen-free extract	675.48	627.43	578.07	521.67	471.60	417.23
DE (Kcal/100 g)^g	403.88	404.21	404.48	403.09	403.14	402.79
P/E (mg CP/Kcal DE)^h	49.65	62.23	74.52	86.87	99.66	112.19

^aTreatment groups having graded levels of protein, i.e., TCP20 (200 g/kg dietary protein), TCP25 (250 g/kg dietary protein), TCP30 (300 g/kg dietary protein), TCP35 (350 g/kg dietary protein), TCP40 (400 g/kg dietary protein), and TCP45 (450 g/kg dietary protein). ^bGNOC: groundnut oil cake, procured from the local market, India. ^cComposition of vitamin-mineral mix (PRE-MIX PLUS) (quantity/kg): vitamin A, 5,500,000 IU; vitamin D3, 1,100,000 IU; vitamin B2, 2,000 mg; vitamin E, 750 mg; vitamin K, 1,000 mg; vitamin B6, 1,000 mg; vitamin B12, 6 mg; calcium pantothenate, 2,500 mg; nicotinamide, 10 g; choline chloride, 150 g; Mn, 27,000 mg; I, 1,000 mg; Fe, 7,500 mg; Zn, 5,000 mg; Cu, 2,000 mg; Co, 450 l-lysine, 10 g; DL-methionine, 10 g; selenium, 125 mg; vitamin C, 2,500 mg. ^dCMC: carboxymethyl cellulose, procured from HiMedia Ltd., India. ^eBHT: butylated hydroxytoluene, procured from HiMedia Ltd., India. ^fStay-C 35, protected vitamin C. ^gDE, digestible energy (Kcal/100 g) = 4 × CP (g/100 g) + 9 × EE (g/100 g) + 4 × NFE (g/100 g) [45]. ^hP/E, protein-to-energy ratio (mg CP/kcal DE) = (CP% ×1000)/DE.