Effects of Varying Dietary Protein Levels on Growth Performance, Survival, Body Composition, Haemato-Biochemical Profile, and Metabolic Responses of Hypselobarbus jerdoni (Day, 1870) Juveniles

Table 9

Carbohydrate and protein metabolic enzyme activity of H. jerdoni juveniles fed diets containing varying levels of dietary protein for the experimental period of 60 days.

Parameters		^aTreatments						SEM	values
		TCP20	TCP25	TCP30	TCP35	TCP40	TCP45		Overall	Linear	Quadratic
GOT^b	GOT muscle	25.19	24.66	20.12	20.89	24.69	21.03	1.22	0.761	0.442	0.618
	GOT liver	18.06^b	18.22^b	15.41^a	15.59^a	18.65^b	18.15^b	0.35	0.000	0.794	0.021
GPT^c	GPT muscle	24.85	26.19	21.84	19.73	27.83	26.23	1.23	0.482	0.719	0.466
	GPT liver	5.16^b	5.33^b	4.52^a	4.61^a	5.37^b	5.09^b	0.09	0.000	0.922	0.042
LDH^d	LDH muscle	9.46^b	9.48^b	6.79^a	6.48^a	6.53^a	6.30^a	0.35	0.000	0.000	0.000
	LDH liver	2.37^b	2.17^b	0.97^a	0.92^a	0.85^a	0.78^a	0.16	0.000	0.000	0.000
MDH^e	MDH muscle	1.53^b	1.61^b	0.62^a	0.64^a	0.56^a	0.50^a	0.12	0.000	0.000	0.000
	MDH liver	0.68	0.81	0.75	0.58	0.78	0.95	0.06	0.710	0.407	0.491

Mean values (n = 3) in the same column with different superscripts differ significantly (). ^aTreatment groups having graded levels of protein, i.e., TCP20, 200 g/kg protein; TCP25, 250 g/kg protein; TCP30, 300 g/kg protein; TCP35, 350 g/kg protein; TCP40, 400 g/kg protein; TCP45, 450 g/kg protein. ^bGOT, glutamate oxaloacetate transaminase activity is expressed as nanomoles of oxaloacetate released/min/mg. ^cGPT, glutamate pyruvate transaminase activity is expressed as nanomoles of sodium pyruvate released/min/mg protein. ^dLDH, lactate dehydrogenase activity is expressed in unit/mg protein/min. ^eMDH, malate dehydrogenase activity is expressed in unit/mg protein/min.