Simultaneous and Direct Determination of Vancomycin and Cephalexin in Human Plasma by Using HPLC-DAD Coupled with Second-Order Calibration Algorithms

Table 1

Determination results of vancomycin and Cephalexin by HPLC-DAD using PARAFAC-ALS and SWATLD algorithm in human plasma (𝑁=4).

Sample	Added		SWATLD		PARAFAC
	Van^c	Cep^d	Van^c	Cep^d	Van^c	Cep^d
	(mg/mL)	(mg/mL)	(mg/mL)	(mg/mL)	(mg/mL)	(mg/mL)
11	15.15	15.27	14.25	14.71	14.02	14.57
12	20.20	20.36	20.58	20.52	20.06	20.49
13	30.30	10.18	30.64	9.99	30.39	10.02
14	20.20	30.54	21.52	29.46	21.50	29.27
15	25.25	25.45	25.24	23.68	24.81	23.71
16	20.20	15.27	21.25	14.39	21.45	14.25
11			94.1%	96.4%	92.5%	95.4%
12			101.9%	100.8%	99.3%	100.7%
13			101.1%	98.1%	100.3%	98.5%
14			106.6%	96.5%	106.5%	95.8%
15			99.9%	93.0%	98.2%	93.2%
16			105.2%	94.2%	106.2%	93.3%
MR^a			102 ± 4%	97 ± 3%	101 ± 5%	96 ± 3%
RMSEP^b			0.0081	0.0095	0.0089	0.0102

^aMean recovery.
^bRoot mean square error of prediction.
^cVancomycin.
^dCephalexin.