Capture-SELEX: Selection of DNA Aptamers for Aminoglycoside Antibiotics

Figure 4

Groups and orphans of oligonucleotides (5′ → 3′) obtained after cloning and sequencing of the single stranded DNA that was selected by the target mixture (kanamycin A disulfate salt dihydrate, sulfacarbamide, sulfamethoxazole, and sotalol hydrochloride, each 1 mM in selection buffer) during 13 Capture-SELEX rounds. The representative of each group is printed in red. Each group could be divided into subgroups of totally identical sequences. The number of sequences in each subgroup is given (qty), and the representative is printed in black. Green: binding site for primer AP60 (18mer), magenta: docking sequence, originally: TGAGGCTCGATC, blue: binding site for primer AP20 (18mer), yellow marking: sites of base exchange or deletion, and underlined: possible G-quadruplex area.