Capture-SELEX: Selection of DNA Aptamers for Aminoglycoside Antibiotics
Figure 5
Specificity tests on the representative of each of the groups of DNA oligonucleotides derived from Capture-SELEX against the mixture of pharmaceuticals. Conditions were chosen comparably to the steps in the SELEX procedure. The four pharmaceuticals were tested individually at a concentration of 1 mM in selection buffer as well as their mixture and selection buffer alone as a negative control. The amount of eluted ssDNA was normalized to 5×107 beads. Experiments were performed in duplicate or triplicate in the case of binding sequences (aptamers), the representatives of groups 1, 4, 6, 7, 8, and 9.
