Review Article
Several Affinity Tags Commonly Used in Chromatographic Purification
Table 1
Common widely used affinity tags for purification of recombinant proteins.
| | Affinity tag | Length (aa) | Size (kDa) | Matrix |
| | Hexahistidine (6x His) | 6 (generally) | 0.84 | Metal ions (Ni2+, Co2+, Cu2+, Zn2+, Fe3+) | | Glutathione S-transferase (GST) | 211 | 26 | Glutathione | | FLAG | 8 | 1.01 | Anti-FLAG mAb | | Streptavidin-binding peptide (SBP) | 38 | 4.3 | Streptavidin | | Strep II | 8 | 1.06 | Strep-Tactin (modified streptavidin) | | Maltose-binding protein (MBP) | 396 | 42 | Amylose | | Calmodulin-binding peptide (CBP) | 26 | 2.96 | Calmodulin | | Chitin-binding domain (CBD) | 51 | 5.59 | Chitin | | S | 15 | 1.75 | S-protein of RNase A | | HA | 9 | 1.1 | Anti-HA epitope mAb | | c-Myc | 11 | 1.2 | Anti-Myc epitope mAb |
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