Portable Bioactive Paper-Based Sensor for Quantification of Pesticides

Figure 2

Two-step sequential reactions of acetylthiocholine (ATCh) for production of yellow colored TNB. In reaction 1, ATCh is broken to acetic acid and thiocholine (TCH). The free sulfhydryl group of TCh is quantified through Ellman’s method in reaction 2. The resulting TNB is used in a direct determination of the activity of ACh esterase.