Solid-Phase Extraction Strategies to Surmount Body Fluid Sample Complexity in High-Throughput Mass Spectrometry-Based Proteomics

Table 1

Various sorbents used in protein SPE.

SPE type	Material	Usage
Reversed-phase	C3, C4, C8	Proteins
	C18	Peptides
Normal phase	ZIC-HILIC	Glycoproteins, glycopeptides
	TSKgel Amide-80	Proteins
	Waters HILIC	Proteins, peptides
	Cotton^*	Glycopeptides, glycans after release from peptides
IEX	WCX, SCX	Proteins
	WAX, SAX	Proteins
Metal-chelating	Ti, Fe	Phosphopeptides
	Ga	Phosphopeptides
	Cu	(Phospho)peptides
Affinity	Lectin	Glycans, glycopeptides
	Boronic acid	Glycans, glycopeptides
	Blue dye	Albumin
	Protein A/G	Immunoglobulins
	Heparin	IgG
	RNA/DNA	Plasmids, DNA binding proteins
	Purine/pyrimidine derivatives	For example, ATP/GTP using enzymes
	Coenzymes	Coenzyme-dependent enzymes
	Vitamins	Vitamin binding proteins
	Antibodies	Proteins, peptides

For further information see [24] and references cited therein.
^*Reference [25].