Determination of Nicotine in Tobacco by Chemometric Optimization and Cation-Selective Exhaustive Injection in Combination with Sweeping-Micellar Electrokinetic Chromatography

Figure 1

The mechanism of CSEI-sweeping-MEKC mode (adapted from [13]). (a) The capillary was rinsed with a nonmicellar, low-pH separation buffer. HCB zone was then introduced hydrodynamically. (b) Cationic samples prepared in low conductive matrix were injected into capillary by positive voltage and concentrated in the HCB zone. (c) The concentrated cationic samples in HCB zone were then swept into a narrow zone by anionic micelles from the inlet sweeping buffer. (d) MEKC separation.

(a)

(b)

(c)

(d)