Determination of Nicotine in Tobacco by Chemometric Optimization and Cation-Selective Exhaustive Injection in Combination with Sweeping-Micellar Electrokinetic Chromatography

Figure 2

Electropherograms of runs 10 (blank line), 13 (red line), and 15 (green line) in CCD. CE conditions: separation buffer: 50 mM phosphate buffer (pH 3) containing methanol; sweeping buffer: 50 mM phosphate buffer (pH 3) containing 20% methanol and SDS; HCB: phosphate buffer (pH 3); applied voltage, −20 kV. Sample injection: 15 kV, 15 min. Nicotine concentration: 617 nM. Other conditions are shown in Table 2.