Determination of Nicotine in Tobacco by Chemometric Optimization and Cation-Selective Exhaustive Injection in Combination with Sweeping-Micellar Electrokinetic Chromatography

Figure 3

Effect of HCB concentration by CSEI-sweeping-MEKC. Separation buffer: 50 mM phosphate buffer (pH 3) containing 20% methanol; sweeping buffer: 50 mM phosphate buffer (pH 3) containing 20% methanol and 50 mM SDS; HCB: phosphate buffer (pH 3); applied voltage, −20 kV. Sample injection: 15 kV, 15 min. Nicotine concentration: 617 nM.