Determination of Nicotine in Tobacco by Chemometric Optimization and Cation-Selective Exhaustive Injection in Combination with Sweeping-Micellar Electrokinetic Chromatography

Figure 4

Electropherograms for determining nicotine (standard solution) under optimized conditions (a) and tobacco sample I (b). CE conditions: separation buffer: 50 mM phosphate buffer (pH 3) containing 25% methanol; sweeping buffer: 50 mM phosphate buffer (pH 3) containing 20% methanol and 50 mM SDS; HCB: 160 mM phosphate buffer (pH 3); applied voltage, −25 kV. Sample injection: 15 kV, 15 min.

(a)

(b)