Determination of Nicotine in Tobacco by Chemometric Optimization and Cation-Selective Exhaustive Injection in Combination with Sweeping-Micellar Electrokinetic Chromatography

Table 2

Experimental design for CCD.

Run	Pattern	Methanol proportion	SDS concentration	HCB phosphate concentration	Peak high (%)
1	− − −	10	50	75	20.38
2	−  − +	10	50	200	40.73
3	− + −	10	125	75	15.73
4	− + +	10	125	200	63.53
5	+ −  −	25	50	75	75.41
6	+ − +	25	50	200	99.02
7	+ + −	25	125	75	71.26
8	+ + +	25	125	200	77.17
9	a 0 0	4.88	87.50	137.50	43.64
10	A 0 0	30.11	87.50	137.50	100
11	0 a 0	17.50	24.43	137.50	56.68
12	0 A 0	17.50	150.56	137.50	71.24
13	0 0 a	17.50	87.50	32.38	14.00
14	0 0 A	17.50	87.50	242.61	48.41
15	0 0 0	17.50	87.50	137.50	52.04
16	0 0 0	17.50	87.50	137.50	48.55