Assessment for Melting Temperature Measurement of Nucleic Acid by HRM

Figure 5

Effect of dNTPs on measurement of DNA duplexes by HRM. The buffer contains 1x EvaGreen, 10 mM phosphate buffer (pH 7.4), and 100 mM NaCl. In this assay, 0, 0.02, 0.2, or 2 mM dNTPs were used.