Analyses of Total Alkaloid Extract of Corydalis yanhusuo by Comprehensive RP × RP Liquid Chromatography with pH Difference

Table 1

Identified alkaloids in C. yanhusuo with MS/MS and UV spectra.

Number	(min); pH 10.5	(min); pH 3.0	(nm)	[M+H]^+ ()	Fragment ions ()	Identified compound
1	13.05	19.01	233, 346	330.3	269.3, 251.7	Tetrahydroprotopapaverine [35]
2	13.55	8.50	229, 265	352.2	337.4, 322.4	13-Methyl-dehydrocorydalmine [36]
3	15.45	2.04	230, 341	366.2	333.9, 279.0	Dehydrocorydaline [37]
4	16.34	6.89	231, 262	328.2	313.0, 279.4	Demethylcorydalmine [35]
5	17.08	14.02	234, 276	320.2	292.3, 275.1	Coptisine [38]
6	22.32	15.48	217, 282	338.2	322.2, 294.3	Columbamine [39]
7	22.60	7.52	242, 273	342.2	278.0, 178.3	Tetrahydrocolumbamine [40]
8	22.88	5.26	224, 282	352.2	306.2, 293.2	13-Methylpalmatrubine [41]
9	24.39	4.73	214, 288	354.2	336.2, 275.2	Protopine [42]
10	25.26	5.41	222, 276	356.2	310.2, 294.0	D-Glaucine [43]
11	26.81	3.53	260, 282	352.2	336.2, 308.2	Dehydrocorybulbine [44]
12	27.06	5.26	220, 280	356.2	191.9, 165.2	D,L-Tetrahydropalmatine [44]
13	28.09	19.01	226, 346	352.2	337.4, 308.2	Palmatine [44]
14	29.16	15.48	220, 266	340.2	324.0, 292.0	Canadine [40]
15	30.18	9.80	245, 374	324.2	307.2, 248.8	D,L-Tetrahydrocoptisine [44]
16	31.64	22.02	209, 285	370.2	354.4, 295.5	Fumaricine [40]