UHPLC-PDA Assay for Simultaneous Determination of Vitamin D₃ and Menaquinone-7 in Pharmaceutical Solid Dosage Formulation

Table 3

Statistical evaluation of regression data of vitamin D₃ and MK-7 by HPLC and UHPLC.

Parameters	Analyte/technique
	HPLC		UHPLC
	Vitamin D3	MK-7	Vitamin D3	MK-7
Linearity range (μg mL^−1)	5–100	5–100	5–100	5–100
Slope	1998606	136363	0.5854	0.5854
Intercept	5725724	70561.4	134.52	104.52
Standard error of slope	92902	6808	3.6 × 10^−2	4.43 × 10^−2
Standard error of intercept	469133	34377	1.181	1.901
Coefficient of determination ()	0.9984	0.9981	0.9984	0.9981
Limit of detection (μg mL^−1)	0.24	0.27	0.16	0.18
Limit of quantification (μg mL^−1)	0.72	0.81	0.48	0.54