Evaluation of Global Genomic DNA Methylation in Human Whole Blood by Capillary Electrophoresis UV Detection

Figure 3

Typical electropherogram of the DNA sample extracted from whole human blood after formic acid hydrolysis obtained with 50 mmol/L BTP phosphate buffer at pH 3.25 and 60 mmol/L sodium acetate buffer at pH 3.60 (4 : 1, v/v) as the run buffer. Electrophoretical conditions: uncoated silica capillary, 60 cm × 100 μm id; cartridge temperature, 18°C; voltage, 22 kV; detection, 280 nm; hydrodynamic injection, 270 nL (2.0 psi × 5 s). Arrow indicates the contaminant peak. Panel shows five consecutive runs of hydrolysed DNA. Cyt = cytosine; mCyt = 5-methylcytosine; Gua = guanosine. Peaks were identified by either co-injection with standards and through the analysis of absorbance spectra.