The Elevation of LC-ESI-Q-TOF-MS Response in the Analysis of Isoquinoline Alkaloids from Some Papaveraceae and Berberidaceae Representatives

Table 2

The influence of spectrometric parameters on the determination of the studied four representatives of isoquinoline alkaloids, expressed in the peak area of the compounds in relation to the highest one in each group (in percent). The most optimum settings for all groups of alkaloids are in italics.

Instrument settings		% BBR	% BBM	% MGN	% SNG
Capillary voltage (V)	A3000	100.00	54.88	100.00	100.00
	A3250	98.77	66.97	92.28	96.25
	A3500	97.19	96.48	90.23	95.17
	A3750	82.83	100.00	81.28	84.77
	A4000	82.80	99.93	77.76	81.96
Fragmentor voltage (V)	75	52.21	62.47	55.49	58.39
	100	70.36	64.59	76.28	66.37
	125	85.44	69.81	95.08	87.35
	150	98.46	95.72	99.95	99.02
	175	100.00	100.00	100.00	100.00
Gas temperatures (°C)	300	99.96	99.94	100.00	98.91
	325	100.00	100.00	99.90	100.00
	350	99.37	99.62	99.92	99.36
	350 and 375 (sheath gas)	97.77	80.09	99.87	95.44
Drying gas and sheath gas flows (L/min)	10 and 10	95.65	92.98	95.09	96.12
	11 and 11	97.55	97.39	99.77	98.30
	12 and 12	100.00	100.00	100.00	100.00
Nebulizer pressure (psig)	25	63.44	77.86	52.95	66.18
	30	75.22	86.90	64.45	78.22
	35	100.00	100.00	98.57	100.00
	40	99.34	99.55	100.00	99.90
Skimmer voltage (V)	40	45.18	47.42	47.96	43.03
	50	86.51	60.99	93.69	81.45
	60	99.38	100.00	95.25	97.76
	65	100.00	98.69	100.00	100.00
	70	92.17	50.10	97.32	98.33