Research Article
Simultaneous Determination of 11 Compounds in Gualou Guizhi Granule and Pharmacokinetics Study by UPLC-MS/MS
Table 4
The precision and accuracy of detected compounds in rat plasma (
).
| | Analyte | Nominal concentration
(μg⋅mL−1) | Intraday precision
/RSD | Interday precision
/RSD | Intraday accuracy | Interday accuracy |
| | Paeoniflorin | 0.1 | 4.98% | 5.52% | 97.35% | 96.93% | | 2 | 4.44% | 3.33% | 92.48% | 92.08% | | 8 | 3.77% | 3.46% | 107.09% | 106.62% | | Albiflorin | 0.1 | 4.28% | 4.01% | 103.05% | 101.85% | | 2 | 2.87% | 4.75% | 108.45% | 101.05% | | 8 | 3.47% | 3.76% | 113.36% | 96.00% | | Liquiritin | 0.01 | 5.53% | 4.68% | 99.15% | 104.48% | | 0.2 | 5.28% | 3.62% | 94.19% | 109.29% | | 0.5 | 3.32% | 4.31% | 97.90% | 105.66% | | Isoliquiritin | 0.01 | 6.12% | 6.18% | 113.25% | 96.76% | | 0.1 | 3.44% | 3.47% | 109.07% | 93.48% | | 0.25 | 3.62% | 4.56% | 99.60% | 108.24% | | Liquirtin apioside | 0.01 | 4.09% | 4.68% | 99.95% | 94.98% | | 0.1 | 5.59% | 4.44% | 114.03% | 90.23% | | 0.25 | 4.38% | 4.42% | 109.95% | 98.40% | | Isoliquiritin apioside | 0.01 | 5.87% | 1.67% | 103.66% | 100.55% | | 0.1 | 2.58% | 3.15% | 98.48% | 95.52% | | 0.25 | 4.07% | 3.87% | 98.95% | 95.14% | | Liquiritigenin | 0.005 | 4.62% | 4.01% | 94.95% | 96.74% | | 0.1 | 4.87% | 5.13% | 94.62% | 94.19% | | 0.2 | 2.57% | 5.14% | 109.56% | 106.41% | | Isoliquiritigenin | 0.005 | 6.41% | 5.85% | 102.95% | 112.04% | | 0.02 | 4.95% | 4.87% | 97.80% | 110.61% | | 0.05 | 5.13% | 4.30% | 94.00% | 91.25% | | Glycyrrhizic acid | 0.1 | 2.21% | 2.84% | 102.05% | 99.15% | | 2 | 4.18% | 3.16% | 96.95% | 91.90% | | 8 | 4.03% | 4.32% | 112.26% | 109.07% | | Glycyrrhetinic acid | 0.01 | 3.58% | 4.01% | 98.59% | 100.15% | | 1 | 5.88% | 4.31% | 108.85% | 111.16% | | 2 | 2.97% | 5.19% | 92.82% | 110.17% | | Paeoniflorin sulfonate | 0.1 | 3.90% | 5.85% | 97.71% | 99.35% | | 2 | 3.54% | 4.72% | 93.66% | 94.38% | | 8 | 3.62% | 5.15% | 107.48% | 96.05% |
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