Research Article
Simultaneous Determination of 11 Compounds in Gualou Guizhi Granule and Pharmacokinetics Study by UPLC-MS/MS
Table 5
The stability of detected compounds in rat plasma (
).
| | Analyte | Nominal concentration
(μg⋅mL−1) | Autosampler stability (24 h) | Stability
(24 h at room temperature) | Freeze-thaw stability (3 cycles) | | Mean | RSD | Mean | RSD | Mean | RSD |
| | Paeoniflorin | 0.1 | 103.46% | 5.06% | 105.66% | 2.67% | 97.71% | 2.01% | | 2 | 98.29% | 5.07% | 91.25% | 4.29% | 92.82% | 4.30% | | 8 | 113.81% | 3.60% | 109.75% | 3.44% | 107.48% | 3.77% |
| | Albiflorin | 0.1 | 97.93% | 3.01% | 94.78% | 1.84% | 99.77% | 4.44% | | 2 | 111.49% | 4.73% | 93.66% | 5.36% | 108.14% | 4.44% | | 8 | 107.72% | 2.72% | 108.45% | 4.47% | 98.89% | 4.68% |
| | Liquiritin | 0.01 | 112.48% | 3.87% | 95.70% | 4.18% | 101.75% | 5.02% | | 0.2 | 92.30% | 3.83% | 110.81% | 3.68% | 109.75% | 4.11% | | 0.5 | 90.84% | 5.13% | 106.73% | 2.84% | 97.91% | 3.02% |
| | Isoliquiritin | 0.01 | 105.18% | 3.83% | 100.74% | 3.18% | 98.98% | 4.61% | | 0.1 | 97.14% | 3.44% | 99.28% | 5.11% | 94.03% | 4.08% | | 0.25 | 95.62% | 4.53% | 98.59% | 3.58% | 108.88% | 2.72% |
| | Liquirtin apioside | 0.01 | 95.66% | 4.46% | 111.24% | 3.77% | 107.20% | 3.24% | | 0.1 | 90.88% | 3.99% | 96.07% | 4.81% | 93.95% | 2.87% | | 0.25 | 105.23% | 4.53% | 104.43% | 4.13% | 94.78% | 4.68% |
| | Isoliquiritin apioside | 0.01 | 102.25% | 5.16% | 101.13% | 3.82% | 109.53% | 4.27% | | 0.1 | 106.88% | 3.81% | 101.45% | 3.31% | 93.01% | 4.87% | | 0.25 | 112.48% | 4.68% | 98.59% | 3.76% | 96.66% | 2.11% |
| | Liquiritigenin | 0.005 | 98.45% | 3.58% | 111.60% | 4.41% | 97.45% | 6.59% | | 0.1 | 93.53% | 5.60% | 108.45% | 4.01% | 93.29% | 5.43% | | 0.2 | 97.16% | 3.17% | 97.03% | 3.73% | 108.02% | 4.68% |
| | Isoliquiritigenin | 0.005 | 106.19% | 5.84% | 99.77% | 5.88% | 99.28% | 3.01% | | 0.02 | 91.71% | 2.78% | 93.66% | 2.58% | 107.70% | 2.82% | | 0.05 | 108.30% | 4.07% | 100.94% | 2.85% | 109.21% | 5.13% |
| | Glycyrrhizic acid | 0.1 | 97.71% | 3.80% | 96.05% | 5.57% | 98.31% | 2.87% | | 2 | 92.82% | 5.28% | 92.18% | 3.68% | 108.78% | 4.87% | | 8 | 107.48% | 4.98% | 94.94% | 5.41% | 98.20% | 4.07% |
| | Glycyrrhetinic acid | 0.01 | 102.25% | 3.35% | 111.03% | 4.54% | 111.93% | 4.95% | | 1 | 97.14% | 3.34% | 95.89% | 3.72% | 92.58% | 2.44% | | 2 | 96.54% | 5.64% | 90.19% | 4.81% | 94.32% | 3.02% |
| | Paeoniflorin sulfonate | 0.1 | 102.25% | 4.44% | 109.21% | 2.23% | 99.57% | 6.05% | | 2 | 96.28% | 4.30% | 94.32% | 5.67% | 94.59% | 4.82% | | 8 | 93.03% | 5.43% | 96.38% | 2.25% | 93.39% | 5.13% |
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