Detection and Structural Characterization of Nucleophiles Trapped Reactive Metabolites of Limonin Using Liquid Chromatography-Mass Spectrometry

Figure 2

Extract ion (m/z 802 → 758) chromatograms obtained from LC-LTQ MS analysis of microsomal incubations containing LIM, GSH, NAL, and NADPH in the absence microsomes (a) or in presence of HLMs (b) or MLMs (c). (d) Extracted ion (m/z 802 → 758) chromatogram obtained from LC-LTQ MS analysis of synthetic M5 and M5′. (e) MS/MS spectrum of M5 generated in microsomal incubations (M5′ showed the same MS/MS spectrum). (f) MS/MS spectrum of synthetic M1 (synthetic M1′ showed the same MS/MS spectrum).

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