Simultaneous Quantitative Determination of Synthetic Cathinone Enantiomers in Urine and Plasma Using GC-NCI-MS

Table 4

Interday and intraday reproducibility results in terms of coefficient of variance for fourteen cathinone-related compounds spiked in urine and plasma at three different concentration levels for the two enantiomers of each compound.

			CV% intraday						CV% interday
			20 µg/L		60 µg/L		100 µg/L		20 µg/L		60 µg/L		100 µg/L
			E1	E2	E1	E2	E1	E2	E1	E2	E1	E2	E1	E2
1	4-FMC	U	3.61	3.11	1.04	1.79	0.41	1.33	10.26	11.85	10.28	12.02	8.14	7.6
		P	6	4.79	2.59	5.36	2.26	2.59	11.01	10.6	10.99	13.3	10.95	12.35
2	4-FEC	U	2.22	5.32	1.75	1.53	2.03	1.69	5.67	10.9	14.69	15.74	15.8	16.13
		P	10.57	11.65	5.31	3.89	1.69	2.21	7.44	8.43	7.59	11.25	6.65	7.76
3	Nor-mephedrone	U	0.7	1.01	2.13	1.64	2	2.17	3.07	4.57	3.5	4.43	1.75	4.91
		P	7.59	7.95	3.07	3.51	2.79	6.97	6.01	7.1	14.13	6.48	14.3	14.21
4	Buphedrone	U	1.42	1.6	1.89	2.28	2.12	1.17	5.6	14.08	15.31	16.91	16.62	15.29
		P	11.13	10.88	3.37	6.27	1.32	3.14	8.04	8.49	13.39	14.26	5	8.32
5	3-MMC	U	3.68	2.03	0.93	1.66	0.39	1.34	12.27	9.02	1.72	2.41	0.82	1.29
		P	9.74	12.68	4.55	6.19	2.51	3.69	8.42	9.85	4.03	4.78	16.3	16.97
6	3-Methylbuphedrone	U	1.36	3.07	2.06	4.48	0.93	1.09	5.32	14.73	6.73	11.96	10.04	9.44
		P	10.58	11.42	5.04	5.44	1.19	3.58	7	7.9	10.55	15.34	13.67	14.31
7	4-Methylbuphedrone	U	1.25	2.16	0.35	0.75	0.68	1.14	3.73	11.28	9.25	2.04	10.22	9.93
		P	9.77	11.67	5.41	4.49	2.42	2.82	9.25	11.85	19.55	6.83	18.03	19.59
8	3-EMC	U	2.38	2.57	0.63	2.1	0.74	1.69	14.53	14.81	13.18	3.51	7.62	10.98
		P	11.36	8.27	5.41	4.55	3.01	3.16	11.35	9.79	19.65	7.13	19.04	17.92
9	3-EEC	U	3.22	6.13	1.13	3.6	1.71	2.13	13.29	15.44	8.42	10.94	3.27	4.1
		P	8.81	12.64	4.3	5.28	3.15	3.71	6.62	9.27	10.19	12.67	14.15	14.82
10	4-EEC	U	3.45	1.85	0.93	2.17	0.84	0.82	14.48	11.31	9.31	12.68	7.19	1.8
		P	11.48	10.53	6.12	4.48	4.24	3.59	8.31	9.46	19.81	19.9	10.97	15.42
11	3,4-DMEC	U	3.7	3.5	1.97	1.02	3.44	2.16	16.54	14.77	10.92	14.49	3.07	2.62
		P	12.11	13.14	4.5	6.31	3.26	3.06	9.44	11.05	18.98	20.31	9.66	15.7
12	2,3-MDMC	U	2.07	3.24	3.16	1.43	4.11	5.56	5.5	4.43	13.57	14.03	5.74	6.6
		P	10.94	7.12	2.74	4.02	7.24	7.13	9.2	5.11	3.41	4.46	19.77	19.35
13	Butylone	U	5.14	6.26	1.36	0.5	0.85	0.88	12.57	14.11	14.56	14.9	1.25	1.56
		P	10.78	12.89	4.97	6.67	2.63	3.25	11.42	13.6	3.66	7.62	9.06	9.14
14	Pentylone	U	7.1	1.34	2.59	1.84	1.83	0.19	12.98	5.99	12.77	14.03	2.29	3.86
		P	10.2	18.64	3.74	5.93	2	2.06	10.29	19.91	9.77	6.67	5.01	6.59

U: urine; P: plasma; CV: coefficient of variance; E1: enantiomer 1; E2: enantiomer 2.