Simple and Selective HPLC-UV/Vis Bioanalytical Method to Determine Aluminum Phthalocyanine Chloride in Skin Permeation Studies

Table 1

Experimental design for robustness assays.

Experiment	Temp (°C)	FR (mL/min)	Acid (mM)
1	29	1.425	0.009
2	31	1.425	0.009
3	29	1.575	0.009
4	31	1.575	0.009
5	29	1.425	0.011
6	31	1.425	0.011
7	29	1.575	0.011
8	31	1.575	0.011

Temp: oven temperature; FR: flow rate; Acid: phosphoric acid concentration of the mobile phase.