Simple and Selective HPLC-UV/Vis Bioanalytical Method to Determine Aluminum Phthalocyanine Chloride in Skin Permeation Studies

Table 2

Optimization of chromatographic conditions for AlPcCl determination.

Trial	Column	Mobile phase (v : v)	Flow rate (mL/min)	Tailing factor	Resolution	Retention time (min)	Peak characteristics
1	RP C18, 15 cm	MeOH : H3PO4 0.01 M (60 : 40)	1.0	—	3.104	2.7	Fronting peak
2	RP C18, 15 cm	MeOH : H3PO4 0.01 M (63 : 37)	1.0	0.813	2.954	2.7	Fronting peak
3	NP, 30 cm	MeOH : H3PO4 0.01 M (60 : 40)	1.0	1.065	1.860	2.5	Early peak
4	NP, 30 cm	MeOH : H3PO4 0.01 M (70 : 30)	1.0	0.947	3.143	2.89	Early peak
5	NP, 30 cm	MeOH : H3PO4 0.01 M (75 : 25)	1.0	0.901	3.881	3.21	Early peak
6	NP, 30 cm	MeOH : H3PO4 0.01 M (80 : 20)	1.0	0.908	5.070	4.77	Suitable peak
7	NP, 30 cm	MeOH : H3PO4 0.01 M (80 : 20)	1.5	0.876	4.475	3.71	Suitable peak