Towards a Dual Lateral Flow Nanobiosensor for Simultaneous Detection of Virus Genotype-Specific PCR Products

Figure 2

Dual lateral flow biosensor optimization studies. Representative lateral flow biosensors and signal intensity graphs for (a) effect of the use of monoclonal (mAnti-fluor) versus polyclonal (pAnti-fluor) anti-fluorescein antibody; (b) effect of the anti-digoxigenin (anti-dig) antibody amount for test zone construction; and (c) effect of the polyclonal anti-fluorescein antibody amount for test zone construction. All tests were performed with dig- and fluor-reference target mixtures. Signal is visualized with anti-biotin Au NPs. TZ-S: anti-digoxigenin zone; TZ-R: anti-fluorescein zone.

(a)

(b)

(c)