Towards a Dual Lateral Flow Nanobiosensor for Simultaneous Detection of Virus Genotype-Specific PCR Products

Figure 3

Effect of anti-biotin functionalized gold nanoparticle amount and signal enhancement with nanoparticle aggregates. Representative lateral flow biosensors and signal intensity graphs for Dig- and Fluor-reference target mixtures. Signal is visualized with (1) 30 nm gold nanoparticles functionalized with anti-biotin antibodies (5 μL); (2) 30 nm Au NPs functionalized with anti-biotin antibodies (10 μL); (3) 30 nm gold nanoparticles functionalized with anti-biotin antibodies (5 μL) and 10 nm anti-BSA conjugated Au NPs (5 μL); and (4) 30 nm gold nanoparticles functionalized with anti-biotin antibodies (5 μL) and 5 nm anti-BSA conjugated NPs (5 μL).