Research Article
Calorimetric Study of Helix aspersa Maxima Hemocyanin Isoforms
Figure 2
Example of the successive annealing process. (a) Complete calorimetric scan of αD+N-HaH in buffer 20 mM HEPES (pH 7.06 at 80°C), at scan rate of 0.5°C min−1 (line A); the first scan of a new sample with the same composition, stopped at 74.5°C (line B); result of subtracting the following (second scan) DSC line from the first one (line C); and the second calorimetric scan stopped at 90°C (line D). (b) Result of the deconvolution of the experimental contour (solid line), which provides two individual components (dash lines).
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