Revising Reverse-Phase Chromatographic Behavior for Efficient Differentiation of Both Positional and Geometrical Isomers of Dicaffeoylquinic Acids

Table 1

Capacity factors (k) of trans- and cis-isomers of diCQAs on different phenyl-derived and C₁₈ column matrices.

Identity		Capacity factors (k′) (min)
		Pinnacle bi-phenyl	Raptor bi-phenyl	Viva bi-phenyl	Phenomenex bi-phenyl	Phenomenex phenyl-hexyl	Pinnacle C18	Raptor C18	Viva C18	Ultra C18
1,3-diCQA	Di-trans	13.324–1.442	14.805–1.436	12.207–1.559	22.629–0.559	22.206–1.078	13.764–1.480	12.420–1.235	11.380–1.487	16.936–1.295
	Mono-cis	14.365–1.442	15.638–1.436	13.276–1.559	23.712–0.559	22.818–1.078	14.104–1.480	13.089–1.235	12.196–1.487	17.292–1.295
	Mono-cis	14.565–1.442	15.901–1.436	13.472–1.559	23.848–0.559	23.029–1.078	14.277–1.480	13.257–1.235	12.535–1.487	—
	Di-cis	19.168–1.442	20.217–1.436	18.112–1.559	25.560–0.559	26.050–1.078	17.523–1.480	17.546–1.235	17.231–1.487	20.918–1.295
1,5-diCQA	Di-trans	19.721–1.442	20.952–1.436	18.541–1.559	25.569–0.559	26.262–1.078	20.299–1.480	18.400–1.235	17.531–1.487	21.619–1.295
	Mono-cis	20.111–1.442	21.052–1.436	—	25.723–0.559	—	18.498–1.480	17.814–1.235	17.154–1.487	21.351–1.295
	Mono-cis	21.198–1.442	21.402–1.436	20.386–1.559	25.855–0.559	—	—	20.101–1.235	19.396–1.487	—
	Di-cis	20.417–1.442	—	—	—	—	18.035–1.480	17.955–1.235	—	—
3,4-diCQA	Di-trans	18.711–1.442	20.178–1.436	17.679–1.559	25.408–0.559	26.697–1.078	18.580–1.480	17.471–1.235	16.994–1.487	21.156–1.295
	Mono-cis	18.102–1.442	19.462–1.436	17.031–1.559	25.691–0.559	—	17.424–1.480	16.523–1.235	16.003–1.487	20.399–1.295
	Mono-cis	20.401–1.442	21.065–1.436	18.987–1.559	—	—	18.901–1.480	18.038–1.235	17.533–1.487	—
	Di-cis	20.984–1.442	21.248–1.436	19.753–1.559	—	—	—	18.706–1.235	18.370–1.487	—
3,5-diCQA	Di-trans	19.322–1.442	20.791–1.436	18.158–1.559	25.539–0.559	25.855–1.078	19.693–1.480	17.963–1.235	17.099–1.487	21.334–1.295
	Mono-cis	19.784–1.442	20.919–1.436	18.471–1.559	25.641–0.559	—	18.089–1.480	17.773–1.235	—	20.846–1.295
	Mono-cis	20.565–1.442	21.156–1.436	19.074–1.559	25.721–0.559	—	18.729–1.480	—	—	21.124–1.295
	Di-cis	—	—	—	—	—	16.877–1.480	16.945–1.235	16.304–1.487	19.561–1.295
4,5-diCQA	Di-trans	21.209–1.442	21.463–1.436	20.379–1.559	25.877–0.559	26.209–1.078	21.341–1.480	20.213–1.235	19.309–1.487	21.766–1.295
	Mono-cis	20.952–1.442	21.338–1.436	19.586–1.559	—	—	20.385–1.480	18.808–1.235	18.025–1.487	21.520–1.295
	Mono-cis	21.906–1.442	21.935–1.436	21.759–1.559	26.330–0.559	26.491–1.078	21.704–1.480	21.369–1.235	21.487–1.487	22.025–1.295
	Di-cis	21.654–1.442	21.702–1.436	21.399–1.559	26.163–0.559	—	20.947–1.480	20.638–1.235	20.200–1.487	—