Revising Reverse-Phase Chromatographic Behavior for Efficient Differentiation of Both Positional and Geometrical Isomers of Dicaffeoylquinic Acids
Table 2
Elution order of the trans- and cis-isomers of diCQAs on different column matrices (phenyl versus alkyl) using aqueous methanol as the eluent post optimization.
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Column chemistry
Pinnacle bi-phenyl
Raptor bi-phenyl
Viva bi-phenyl
Phenomenex bi-phenyl
Phenomenex phenyl-hexyl
Pinnacle C18
Raptor C18
Viva C18
Ultra C18
Elution order of isomers
1,3-diCQA
TM M#C
TM M#C
TM M#C
TM M#C
TM M#C
TM M#C
TM M#C
TM M#C
TM M#C
1,5-diCQA
TM CM#
TM CM#
TM CM#
TM M#
TM
CM T
M CTM#
M TM#
CM TM#
3,4-diCQA
M TM#C
M TM#C
M TM#C
TM C
M TM#
M TCM#
M TM#C
M TM#C
M TM#
3,5-diCQA
TCM M#
TCM M#
TCM M#
TM M#
T
CM M#T
CM T
CTM#
CM M#T
4,5-diCQA
M TCM#
M TCM#
M TCM#
TM CM#
M TM#
M CTM#
M TCM#
M TCM#
M CTM#
T represents the di-trans-isomer; M represents the first eluting mono-cis-isomer; M# represents the second eluting mono-cis-isomer; C represents the di-cis-isomer.