Research Article

Revising Reverse-Phase Chromatographic Behavior for Efficient Differentiation of Both Positional and Geometrical Isomers of Dicaffeoylquinic Acids

Table 2

Elution order of the trans- and cis-isomers of diCQAs on different column matrices (phenyl versus alkyl) using aqueous methanol as the eluent post optimization.

 Column chemistry
Pinnacle bi-phenylRaptor bi-phenylViva bi-phenylPhenomenex bi-phenylPhenomenex phenyl-hexylPinnacle C18Raptor C18Viva C18Ultra C18

Elution order of isomers1,3-diCQATM M#CTM M#CTM M#CTM M#CTM M#CTM M#CTM M#CTM M#CTM M#C
1,5-diCQATM CM#TM CM#TM CM#TM M#TM CM TM CTM#M TM#CM TM#
3,4-diCQAM TM#CM TM#CM TM#CTM CM TM#M TCM#M TM#CM TM#CM TM#
3,5-diCQATCM M#TCM M#TCM M#TM M#TCM M#TCM TCTM#CM M#T
4,5-diCQAM TCM#M TCM#M TCM#TM CM#M TM#M CTM#M TCM#M TCM#M CTM#

T represents the di-trans-isomer; M represents the first eluting mono-cis-isomer; M# represents the second eluting mono-cis-isomer; C represents the di-cis-isomer.