Simultaneous Analysis of Twelve Bile Acids by UPLC-MS and Exploration of the Processing Mechanism of Bile Arisaema by Fermentation

Table 6

Recoveries of the twelve compounds (n = 3).

Analytes	Samples (g)	Origin (μg)	Spiked (μg)	Found (μg)	Mean recovery (%) (RSD, %)
(1) THCA	1.0	52.13	42.72	94.41	99.23 (1.11)
			53.40	104.52	98.27 (1.79)
			64.08	115.56	97.10 (2.96)
(2) THDCA	1.0	103.35	82.69	185.95	99.90 (1.95)
			103.36	205.63	98.93 (2.43)
			124.03	227.24	95.36 (2.10)
(3) TCA	1.0	70.48	56.82	126.41	98.73 (2.30)
			71.02	143.35	97.87 (1.46)
			85.22	154.87	99.07 (2.13)
(4) GHCA	1.0	28.20	22.40	50.42	99.13 (0.96)
			28.00	56.05	96.30 (1.97)
			33.60	61.37	98.63 (2.49)
(5) GCA	1.0	28.35	23.04	51.42	100.63 (0.66)
			28.80	56.53	97.90 (1.77)
			34.56	62.50	96.17 (0.96)
(6) GHDCA	1.0	264.26	211.68	476.52	100.33 (0.90)
			264.60	523.14	97.87 (1.76)
			317.52	580.71	98.67 (2.30)
(7) TCDCA	1.0	270.41	219.00	488.12	96.43 (1.99)
			273.75	544.43	102.15 (0.65)
			328.50	593.45	98.30 (1.99)
(8) HCA	1.0	31.11	24.96	55.63	98.43 (1.12)
			31.20	62.12	97.10 (1.68)
			37.44	68.41	99.73 (2.21)
(9) CA	1.0	28.71	22.88	51.34	97.10 (1.36)
			28.60	57.18	98.73 (1.57)
			34.32	62.56	100.97 (1.53)
(10) GCDCA	1.0	651.34	520.08	1161.43	98.13 (1.48)
			650.10	1301.54	100.15 (1.45)
			780.12	1434.82	101.40 (1.08)
(11) HDCA	1.0	21.62	16.72	38.43	96.33 (0.85)
			21.12	42.71	98.73 (1.42)
			24.64	46.08	99.17 (0.44)
(12) CDCA	1.0	127.84	101.40	228.02	99.17 (0.78)
			126.75	253.71	97.60 (1.06)
			152.10	279.73	101.07 (1.50)