Simultaneous Determination of Five Chromones of Radix Saposhnikoviae Extract in Rat Plasma by UPLC-MS/MS: Application to a Comparative Pharmacokinetic Study in Normal and Febrile Rats

Table 3

Precision, accuracy, extraction recovery, and matrix effect of the analytes ().

Analytes	Intraday		Interday		Recovery		Matrix effect
Concentration (μg/mL)	Precision (RSD%)	Accuracy (RE%)	Precision (RSD%)	Accuracy (RE%)	Mean ± SD (%)	RSD (%)	Mean ± SD (%)	RSD (%)
Prim-O-glucosylcimifugin
0.008	2.38	−1.18	1.46	−0.14	94.73 ± 4.41	4.65	93.25 ± 4.44	4.77
0.024	4.70	1.37	3.19	−5.50	93.35 ± 5.00	5.36	89.19 ± 4.45	4.98
0.238	2.91	−4.80	3.08	−2.81	93.03 ± 3.76	4.04	92.75 ± 1.91	2.06
2.378	2.89	−2.63	5.67	5.53	91.40 ± 4.35	4.76	90.35 ± 5.28	5.85
Cimifugin
0.033	2.75	−0.45	2.67	−0.93	95.07 ± 3.90	4.10	96.93 ± 4.43	4.57
0.099	1.99	−2.68	3.03	0.69	90.72 ± 3.73	4.11	91.28 ± 2.93	3.20
0.992	2.51	2.32	3.22	−1.12	89.52 ± 4.73	5.29	86.50 ± 3.80	4.39
9.915	4.67	−1.69	5.31	−2.09	97.65 ± 2.89	2.75	98.37 ± 4.59	4.67
4′-O-β-D-glucosyl-5-O-methylvisamminol
0.003	2.95	−2.59	4.13	−1.30	95.09 ± 4.98	5.23	98.30 ± 3.53	3.58
0.009	2.37	−4.10	2.57	2.47	93.66 ± 4.23	4.51	95.14 ± 3.96	4.16
0.093	1.31	−2.57	2.40	−1.04	88.75 ± 2.26	2.55	92.65 ± 1.96	2.12
0.926	6.87	5.14	1.60	−1.57	94.35 ± 4.66	4.94	89.75 ± 2.29	2.56
5-O-methylvisamminol
0.011	4.20	2.98	2.83	2.37	94.37 ± 3.48	3.68	97.28 ± 3.46	3.56
0.033	5.33	−3.63	3.04	−4.25	91.15 ± 5.43	5.96	102.18 ± 3.86	3.78
0.333	4.23	4.33	2.82	−1.88	93.33 ± 5.44	5.82	89.30 ± 2.75	3.08
3.325	3.94	−3.4	3.02	0.35	95.87 ± 3.20	3.34	95.03 ± 4.84	5.09
Sec-O-glucosylhamaudol
0.001	5.49	−2.78	5.13	−2.22	95.14 ± 4.47	4.70	98.23 ± 4.41	4.49
0.004	3.72	−4.88	3.58	1.46	93.05 ± 3.36	3.61	101.33 ± 2.97	2.93
0.041	2.82	−3.46	2.84	−1.15	97.03 ± 4.33	4.46	96.73 ± 6.37	6.58
0.414	3.18	1.04	3.57	−2.17	88.26 ± 5.91	6.69	102.85 ± 4.22	4.10
Puerarin (IS) 0.100					91.21 ± 5.82	4.19	101.88 ± 3.74	2.62