Simultaneous Determination of Five Chromones of Radix Saposhnikoviae Extract in Rat Plasma by UPLC-MS/MS: Application to a Comparative Pharmacokinetic Study in Normal and Febrile Rats

Table 4

The stability test of the analytes in rat plasma ().

Analytes	At room temperature for 12 h		After three freeze-thaw cycles		At −80°C for 14 days		Post-treatment for 12 h at 4°C
Concentration (μg/mL)	RSD (%)	RE (%)	RSD (%)	RE (%)	RSD (%)	RE (%)	RSD (%)	RE (%)
Prim-O-glucosylcimifugin
0.024	3.64	−3.20	7.40	3.70	2.13	−4.78	1.61	−3.97
0.238	3.06	−3.93	6.06	2.88	2.26	−5.83	1.49	−4.17
2.378	2.10	−1.38	1.86	−2.34	2.54	−1.49	4.36	2.51
Cimifugin
0.099	3.72	1.46	4.75	−4.03	5.19	−3.84	3.29	−3.71
0.992	3.79	−1.86	4.35	−4.49	3.24	1.20	4.88	−1.66
9.915	4.24	−1.38	6.62	−2.26	3.22	1.06	3.74	−3.82
4′-O-β-D-glucosyl-5-O-methylvisamminol
0.009	2.05	−4.07	2.36	−3.11	3.03	−4.22	1.16	0.86
0.093	1.86	4.17	2.68	−6.72	1.51	−5.96	4.34	−5.01
0.926	1.58	−2.29	2.04	2.47	2.87	−3.15	3.05	−2.64
5-O-methylvisamminol
0.033	3.49	−2.78	4.41	−4.35	2.69	−3.34	2.83	1.36
0.333	2.96	4.94	3.93	−4.89	1.62	5.49	4.13	−3.00
3.326	3.73	−2.96	5.08	−3.13	2.59	2.17	3.48	−3.05
Sec-O-glucosylhamaudol
0.004	2.21	−2.17	4.21	−4.39	5.24	2.85	3.63	−5.34
0.041	4.13	−1.18	3.83	2.09	4.82	−3.53	5.96	−4.77
0.414	3.90	−1.71	2.26	1.06	3.70	−1.85	4.73	−2.19