Research Article

Comparison of Phenolic Compound Separations by HPTLC and PPEC with SDS as the Mobile Phase Component

Figure 5

Migration distance of phenolic acids vs. mobile phase buffer pH in the mobile phase for HPTLC (a) and PPEC (b) experiments. The mobile phase: butanol (0.89 M; 8.14% v/v), 10% acetonitrile, 0.1144 mM SDS, and aqueous buffer (citric acid, tris(hydroxymethyl)aminomethane, glycine 0.8 mM titrated to proper pH with KOH or HCl). The stationary phase: HPTLC RP-18W. TLC: experiment time 20 min. PPEC: polarization voltage 800 V; experiment time 8 min.
(a)
(b)