Research Article
Identification and Quality Evaluation of Raw and Processed Asarum Species Using Microscopy, DNA Barcoding, and Gas Chromatography-Mass Spectrometry
Table 3
Regression equation, linear range, correlation coefficients (r2), LLOQ, precision, repeatability, stability, and recovery of 10 investigated analytes (n = 6).
| | Compounds | Regression equation | Linear range (μg/mL) | r2 | LLOQ (μg/mL) | Precision RSD (%) | Repeatability RSD (%) | Stability RSD (%) | Recovery (%) |
| | (1R)-(+)-alpha-Pinene | y = 110696.5x − 19785.11 | 0.08–64 | 0.999 | 0.03 | 3.4 | 4.5 | 2.2 | 94.8 | | (−)-β-Pinene | y = 103513.6x − 18753.84 | 0.1–80 | 0.999 | 0.05 | 4.0 | 4.9 | 2.7 | 105.7 | | (+)-Car-3-ene | y = 81430.83x + 6001.557 | 0.1–80 | 0.999 | 0.02 | 4.2 | 4.4 | 2.5 | 102.6 | | Cineole | y = 31324.58x + 51269.66 | 0.25–200 | 0.998 | 0.03 | 4.3 | 8.9 | 4.1 | 95.5 | | (−)-Borneol | y = 99069.16x − 5912.898 | 0.1–80 | 0.999 | 0.02 | 3.4 | 6.1 | 2.6 | 108.1 | | 2,6,6-Trimethylcyclohepta-2,4-dien-1-one | y = 51336.33x + 33969.81 | 0.12–96 | 0.999 | 0.02 | 6.1 | 4.5 | 5.4 | 94.5 | | α-Terpineol | y = 61057.91x − 10884.64 | 0.05–40 | 0.999 | 0.04 | 6.0 | 8.8 | 6.6 | 89.2 | | Estragole | y = 49990.04x + 171669.0 | 0.3–240 | 0.996 | 0.01 | 1.8 | 6.5 | 5.5 | 102.3 | | Safrole | y = 35569.62x + 225493.1 | 0.6–480 | 0.997 | 0.02 | 4.0 | 5.5 | 3.6 | 111.3 | | Methyleugenol | y = 51970.14x + 466412.8 | 0.6–480 | 0.994 | 0.01 | 5.1 | 2.3 | 5.1 | 101.2 |
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