Bioguided Isolation and Structure Identification of Acetylcholinesterase Enzyme Inhibitors from Drynariae Rhizome

Figure 4

AChE activity determination by UPLC-MS/MS. Representative MRM chromatograms of (a) blank mixture incubated without the substrate, (b) a spike of Ch-I in a tris-buffer, and (c) Ch-I in the sample after the enzymatic reaction.

(a)

(b)

(c)