Simultaneous Determination of 5 Components in the Leaves of Dimocarpus longan by Quantitative Analysis of Multicomponents by Single Marker (QAMS) Based on UPLC and HPLC

Table 3

Robustness test of ƒ_s/i.

Influencing factors		RCFs
		UPLC	Column: Waters	0.397	0.479	0.817	0.902
Column: Thermo	0.398		0.479	0.824	0.901
Flow rate: 0.2 ml/min	0.397		0.481	0.812	0.897
Flow rate: 0.3 ml/min	0.391		0.479	0.803	0.889
Column temperature: 25°C	0.393		0.487	0.808	0.925
Column temperature: 30°C	0.396		0.481	0.809	0.895
Column temperature: 35°C	0.399		0.482	0.818	0.901
HPLC	Column: Phenomenex	0.376	0.471	0.785	0.879
	Column: Agilent	0.377	0.471	0.775	0.893
RSD (%)		2.28	1.06	2.07	1.48