Pharmacokinetic Comparisons of Eight Active Components from Raw Farfarae Flos and Honey-Processed Farfarae Flos after Oral Administration in Rats by UHPLC-MS/MS Approaches

Table 1

Optimized SRM parameters of 8 analytes and IS.

Peak no.	Analytes	Retention time (min)	Precursor ion ([M − H]^−) (m/z)	Product ion (m/z)	Collision energy (V)	Polarity
1	Ferulic acid	13.05	193.00	134.00; 177.97	27.93; 13.03	Negative
2	Caffeic acid	6.11	178.96	107.0; 135.0	22.66; 15.53	Negative
3	Chlorogenic acid	5.65	353.04	126.92; 190.97	34.30; 16.75	Negative
4	Neochlorogenic acid	2.94	353.04	178.97; 190.97	18.23; 18.87	Negative
5	Cryptochlorogenic acid	6.14	353.04	173.04; 178.97	15.65; 16.26	Negative
6	Isochlorogenic acid B	12.86	515.00	173.05; 353.07	26.91; 18.98	Negative
7	Isochlorogenic acid C	14.40	515.10	172.97; 353.16	28.35; 17.92	Negative
8	Rutin	13.17	610.00	300.04; 301.01	36.35; 34.64	Negative
IS	Chloramphenicol	10.85	321.00	152.07; 257.07	16.26; 16.22	Negative