Pharmacokinetic Comparisons of Eight Active Components from Raw Farfarae Flos and Honey-Processed Farfarae Flos after Oral Administration in Rats by UHPLC-MS/MS Approaches

Table 4

Matrix effects and extraction recoveries for the analytes in rat plasma.

Analytes	Concentration (ng/mL)	Matrix effect (%, mean ± SD, n = 6)	Recovery (%, mean ± SD, n = 6)
Ferulic acid	100.00	91.70 ± 3.26	102.70 ± 4.97
	200.00	88.68 ± 2.80	100.49 ± 2.41
	500.00	98.99 ± 5.31	99.04 ± 4.10
Caffeic acid	380.00	81.94 ± 3.24	104.48 ± 5.80
	3800.00	85.03 ± 9.17	81.54 ± 3.06
	6000.00	95.54 ± 6.83	95.55 ± 7.83
Chlorogenic acid	3500.00	91.35 ± 4.79	100.07 ± 5.27
	40400.00	99.34 ± 7.87	99.48 ± 3.17
	110000.00	95.09 ± 4.47	95.81 ± 2.29
Neochlorogenic acid	1000.00	95.75 ± 4.72	90.14 ± 4.73
	15000.00	99.46 ± 8.90	99.29 ± 5.83
	25000.00	99.63 ± 3.71	99.28 ± 3.32
Cryptochlorogenic acid	2000.00	97.98 ± 2.49	97.94 ± 6.42
	50000.00	101.71 ± 3.20	99.30 ± 7.60
	100000	100.32 ± 6.42	97.65 ± 4.42
Isochlorogenic acid B	2000.00	101.26 ± 6.32	99.13 ± 7.56
	80000.00	99.45 ± 12.51	99.83 ± 4.23
	160000.00	99.88 ± 4.49	103.60 ± 3.72
Isochlorogenic acid C	1500.00	103.02 ± 5.12	100.54 ± 3.38
	7500.00	99.03 ± 7.34	98.80 ± 4.49
	140000.00	100.43 ± 4.79	90.31 ± 5.51
Rutin	600.00	99.57 ± 4.76	102.72 ± 3.55
	4000.00	98.70 ± 3.57	100.86 ± 6.63
	8000.00	99.11 ± 4.95	101.40 ± 4.63
Chloramphenicol (IS)	100.00	90.77 ± 6.66	92.85 ± 4.67