Simultaneous Determination of 34 Amino Acids in Tumor Tissues from Colorectal Cancer Patients Based on the Targeted UHPLC-MS/MS Method

Table 2

The optimized MRM parameters of 34 amino acids and 3 ISs (ESI positive).

Analyte	Molecular weight	Precursor ion	Product ion	Fragmentor (V)	Collision energy (eV)
Gly	75.07	76	30	50	13
Ala	89.09	90	44	50	8
Ser	105.09	106	60	65	9
Val	117.15	118	72	60	7
Thr	119.12	120	74	65	9
Leu	131.17	132	86	65	7
Ile	131.17	132	86	65	7
Asp	133.10	134	74	65	12
Lys	146.19	147	84	70	11
Gln	146.14	147	84	65	12
Glu	147.13	148	84	70	12
Phe	165.19	166	120	65	10
Arg	174.20	175	70	90	16
Tyr	181.19	182	136	70	11
Pro	115.13	116	70	70	15
Asn	132.12	133	74	60	11
Met	149.21	150	56	65	13
Trp	204.23	205	188	70	5
Cys	121.16	122	59	60	21
His	155.15	156	110	80	13
Cit	175.19	176	159	70	7
ADMA	202.25	203	46	90	16
Cyss	240.30	241	152	80	12
Sar	89.09	90	44	55	10
Apa	89.09	90	30	60	12
Amp	103.12	104	30	60	11
Aba	103.12	104	87	65	9
Opr	129.11	130	84	75	12
Hpr	131.13	132	86	75	13
Orn	132.16	133	70	65	10
Ahd	161.16	162	98	65	11
Hia	179.17	180	105	65	6
SDMA	202.25	203	172	90	14
Kyn	208.21	209	192	70	7
Ala-d4	93.12	94	48	50	9
Met-d3	152.23	153	56	65	13
Phe-d5	170.22	171	125	70	11