Journal of Analytical Methods in Chemistry

Research Article

Simultaneous Determination of 34 Amino Acids in Tumor Tissues from Colorectal Cancer Patients Based on the Targeted UHPLC-MS/MS Method

Table 4

The retention times, regression equations, coefficients, calibration ranges, and LLOQ for 34 amino acids and each corresponding IS.


Analyte	Retention time (min)	Regression equation (n = 9)	Coefficient R²	Calibration range (ng/ml)	LLOQ (ng/ml)	IS

Gly	1.866	y = 1.973405 × 10⁻⁵x + 0.013171	0.99434	1000∼80000	1000	Phe-d5
Ala	2.118	y = 0.002292x − 0.471134	0.99538	1000∼80000	1000	Ala-d4
Ser	1.809	y = 1.120434 × 10⁻⁴x + 0.405218	0.99220	1000∼80000	1000	Phe-d5
Val	3.607	y = 0.003533x − 0.598212	0.99472	1000∼80000	1000	Ala-d4
Thr	2.026	y = 0.001422x + 0.129849	0.99604	1000∼80000	1000	Met-d3
Leu	6.402	y = 0.021977x − 7.784039	0.99375	1000∼80000	1000	Met-d3
Ile	5.863	y = 0.022788x − 6.270995	0.99535	1000∼80000	1000	Met-d3
Asp	1.787	y = 4.507537 × 10⁻⁴x + 0.522395	0.98923	1000∼80000	1000	Met-d3
Lys	2.994	y = 5.106616 × 10⁻⁴x + 0.021605	0.98157	1000∼80000	1000	Phe-d5
Gln	1.882	y = 0.005491x + 4.126505	0.99513	1000∼80000	1000	Met-d3
Glu	2.066	y = 0.001872x + 0.886773	0.99897	1000∼80000	1000	Met-d3
Phe	7.554	y = 0.006928x − 3.149161	0.99258	1000∼80000	1000	Phe-d5
Arg	3.329	y = 0.001577x + 0.298584	0.99370	1000∼80000	1000	Met-d3
Tyr	4.427	y = 1.241443 × 10⁻⁴x + 0.056822	0.99250	1000∼80000	1000	Phe-d5
Pro	2.146	y = 0.004142x + 0.052886	0.99646	500∼40000	500	Ala-d4
Asn	1.770	y = 9.372446 × 10⁻⁴x + 0.471997	0.99304	500∼40000	500	Met-d3
Met	3.636	y = 0.001404x − 0.179621	0.99310	500∼40000	500	Met-d3
Trp	8.755	y = 3.881832 × 10⁻⁴x − 0.034688	0.99746	500∼40000	500	Phe-d5
Cys	2.056	y = 6.151545 × 10⁻⁴x − 0.024106	0.99544	100∼8000	100	Met-d3
His	2.695	y = 0.005120x + 0.129674	0.98902	100∼8000	100	Met-d3
Cit	2.139	y = 0.00316x + 0.123514	0.99490	100∼8000	100	Met-d3
ADMA	3.610	y = 2.22466 × 10⁻⁴x − 0.008033	0.99537	100∼8000	100	Phe-d5
Cyss	1.843	y = 3.122902 × 10⁻⁵x + 0.007852	0.99611	100∼8000	100	Phe-d5
Sar	1.856	y = 7.269948 × 10⁻⁴x + 0.017312	0.99724	50∼4000	50	Phe-d5
Apa	2.432	y = 7.290745 × 10⁻⁴x − 0.009428	0.99649	50∼4000	50	Met-d3
Amp	3.181	y = 1.459612 × 10⁻⁴x + 8.722727 × 10⁻⁴	0.98729	50∼4000	50	Phe-d5
Aba	2.771	y = 0.005955x − 0.085380	0.99898	50∼4000	50	Met-d3
Opr	2.567	y = 1.048082 × 10⁻⁴x + 0.035973	0.99634	50∼4000	50	Phe-d5
Hpr	1.759	y = 0.017388x + 0.017864	0.99533	50∼4000	50	Met-d3
Orn	2.676	y = 0.001756x + 0.236473	0.99406	50∼4000	50	Met-d3
Ahd	2.577	y = 0.003022x − 0.039314	0.99902	50∼4000	50	Ala-d4
Hia	7.210	y = 0.001064x − 0.002872	0.99309	50∼4000	50	Phe-d5
SDMA	3.619	y = 7.281465 × 10⁻⁵x − 0.001614	0.99514	50∼4000	50	Phe-d5
Kyn	6.298	y = 3.698408 × 10⁻⁴x − 4.537424 × 10⁻⁴	0.99744	50∼4000	50	Phe-d5