Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction

<div>Electropherogram of target gene fragment in HCR. The lanes on both sides of the gel are DNA markers; lanes 1–6 represent six soybean cultivars in sequence: Wuhei, Jinda 73, Jinyi 30, Jinke 4, WS 285, and Jinda 47 in HCR system. (a) Double-strand target gene (TF/TR) amplified by PCR. (b) Agarose gel analysis of the PCR product digested by lambda exonuclease. (c) Agarose gel analysis of HCR products in the presence of probes GAa1 and GAa2. (d) Agarose gel analysis of HCR products in the presence of probes GAb1 and GAb2.</div>

Journal of Analytical Methods in Chemistry

fig2

Figure 2

Figure 2: Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction 