Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction

Figure 4

Visual-chip based detection of GmSg-1. Each circle was coated with a mixture of HCR systems containing different hairpin probes and target genes with different sequences. Circles 1 and 4: the probes in the system were replaced with sterile ultrapure water as blank; target genes were GAa1/GAa2 and GAb1/GAb2, respectively. Circles 2 and 3: the probes in the system were all GAa1/GAa2; the target genes were TAa and Tab, respectively. Circles 5 and 6: the probes in the system were all GAb1/GAb2; the target genes were TAa and Tab, respectively.