Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction

Table 1

Probes and target fragments required for HCR reactions.

Sequence name	Gmsg-1 gene probe sequence (5′ to 3′)
TA92F	5′-ATCCCACGACTTGCCTTCA-3′
TA92R	5′-CCCGTGTCGGAATGGAGT-3′
GAa1	5′-CCTCTTCGCCGTCAGCGCCATGAAGTCCGTGGGTAGGGCGGGTTGGGAAATTACCCACTTCATGGCGCTGACGGCG-3′
GAa2	5′-CGGACTTCATGGCGCTGACGGCGAAGAGGCGCCGTCAGCGCCATGAAGTCGGTGGGTA-3′
TAa	5′-CGGACTTCATGGCGCTGACGGGGTGAGATATG-3′
TAa92	5′-CCCGTGTCGGAATGGAGTTCGGGGTGAGATATGACGGACTTCATGGCGCTGACGGCGAAGAGGGGGTATGAGTTGAAGGCAAGTCGTGGGAT-3′
GAb1	5′-CTCTTCTCCGGCGCCGCCATGAAGTGCGTGGGTAGGGCGGGTTGGGAAATTACCCACCCACTTCATGGCGGCGCCGGAG-3′
GAb2	5′-GCACTTCATGGCGGCGCCGGAGAAGAGG-3′
TAb	5′-GCACTTCATGGCGGCGCCGGAGAAGAGG-3′
TAb92	5′-CCCGTGTCGGAATGGAGTTCGGGGTGAGATATGACGCACTTCATGGCGGCGCCGGAGAAGAGGGGGTATGTTGAAGGCAAGTGGGAT-3′