Quality Assessment of Different Species and Differently Prepared Slices of Zedoray Rhizome by High-Performance Liquid Chromatography and Colorimeter with the Aid of Chemometrics

Table 2

The results of methodology validation for HPLC analysis.

Analytes	Calibration curves	Range (μg·mL^−1)	LOD (μg·mL^−1)	LOQ (μg·mL^−1)	Recovery (%)	Precision (%)			Stability (%)
					Mean	RSD	Intraday	Interday	RSD
Curcumenol	y = 1.0 × 10^7x − 56762 (R^2 = 0.9993)	0.0037∼0.75	0.18	0.60	101.1	3.5	1.9	0.52	1.6
Curzerene	y = 2.0 × 10^7x − 15711 (R^2 = 0.9992)	0.0075∼1.88	0.06	0.21	99.24	2.4	2.5	1.3	2.0
Curdione	y = 2.0 × 10^7x − 50917 (R^2 = 0.9998)	0.0072∼2.71	0.15	0.50	100.4	1.8	0.28	1.6	1.8
Isourecumenol	y = 2.0 × 10^7x − 11663 (R^2 = 0.9997)	0.00032∼0.32	0.07	0.22	100.3	4.4	0.24	0.99	1.1
Furanodienon	y = 4.0 × 10^7x − 6308 (R^2 = 0.9998)	0.0069∼2.60	0.03	0.11	100.5	3.1	0.25	1.2	1.6
Curcumol	y = 2.0 × 10^7x − 193973 (R^2 = 0.9999)	40.00∼3590	0.17	0.57	99.98	2.1	0.23	0.97	1.2
Germacrone	y = 2.0 × 10^7x + 812374 (R^2 = 0.9998)	205.00∼1435	0.05	0.16	99.42	2.0	0.78	1.3	1.4
Furanodiene	y = 4.0 × 10^7x − 19611 (R^2 = 0.9999)	3.26∼1630.5	0.05	0.18	98.77	1.5	0.31	0.82	1.6
β-Elemene	y = 8.0 × 10^6x − 21191 (R^2 = 0.9999)	1.72∼724.5	0.33	1.08	99.95	1.9	1.8	1.4	2.1
Bisdemethoxycurcumin	y = 6.3 × 10^5x − 4131.6 (R^2 = 0.9998)	0.35∼4.16	0.06	0.11	98.57	2.0	0.45	1.4	0.49
Demethoxycurcumin	y = 9.8 × 10^5x − 2.2 × 10^5 (R^2 = 0.9999)	0.72∼14.92	0.12	0.15	98.94	1.3	0.19	1.5	0.37
Curcumin	y = 1.3 × 10^6x − 22070 (R^2 = 0.9998)	0.26∼31.68	0.01	0.02	99.78	1.8	0.19	1.1	0.42