Determination of the Contents of Antioxidants and Their Degradation Products in Sodium Chloride Injection for Blood Transfusion

Figure 5

The typical HPLC chromatograms of 12 standard solutions under the optimized chromatographic conditions. The column temperature was 35°C (Waters SymmetryShield TM RP18 column, 5 μm, 250 × 4.6 mm); the injection volume was 10 μL; the UV detection wavelength was 277 nm; the flow rate was 1.0 mL/min; the mobile phase A was acetonitrile; mobile phase B was methanol; and mobile phase C was HAc-water (1 : 99, v/v). Peaks: (1) BHT-OH, (2) BHT-COOH, (3) 1310, (4) BHT-CHO, (5) BHT-Q, (6) DBP, (7) BHT, (8) 3114, (9) 1010, (10) 1330, (11) 1076, and (12) 168.